The ‘One Health’ approach has been promoted and rolled out across the Caribbean region. A Caribbean One Health policy was written and then approved by the Caribbean Community (CARICOM). In parallel, a six-year One Health strategic framework mapping out the implementation of the One Health policy was written and distributed to Caribbean countries and international organisations. 29 inter-sectoral One Health leaders from 12 countries were trained in all areas of One Health. They created national One Health networks and developed and conducted a community-based One Health national project. Using a train-the-trainer philosophy, over 500 field veterinarians and ‘first responders’ were trained in the recognition and response to key exotic, endemic and zoonotic diseases. Laboratory capacity and capability was also strengthened in veterinary diagnostic laboratories across the region through Quality Assurance & Biosafety training, proficiency testing and the introduction of novel molecular diagnostic techniques into laboratories.