Over 50 scientific staff from 14 countries of the Southern African Development Community (SADC) were trained on in situ conservation methodologies, predictive characterisation and pre-breeding of crop wild relatives to better conserve and use these resources. A toolkit on conservation planning of crop wild relatives, templates and tools for preparing National Strategic Action Plans for the conservation and use of crop wild relatives as well as National Strategic Action Plans for Mauritius, South Africa and Zambia were developed. The diversity of crop wild relatives was documented and assessed, priority species for conservation and areas for in situ conservation were identified, and concrete and strategic actions for their conservation and use delineated. Together with farmers in Zambia, incentive mechanisms for their conservation were developed and conservation costs determined. A concept for a regional in situ crop wild relatives conservation strategy was developed for the SADC region.