Disease assessment surveys for tomato, cowpea and pumpkin were carried out in Trinidad and Tobago, St. Vincent and Grenadines and Guyana: disease incidences were recorded and new diseases identified. For full proof identification of diseases, several classical and advanced DNA-based diagnostic methods were developed and optimised for all the pathogens observed. Integrated Disease Management (IDM) methods were then developed and demonstrated in farmer’s fields, and were found to be more economical and environmentally sustainable compared to conventional disease management methods. The disease diagnosis methods and IDM practices were transferred to agricultural officers, researchers, lead farmers and representatives of NGOs involved in sustainable agriculture in the Caribbean. The Plant-Microbe research laboratory at UWI continues to assist vegetable growers, the Ministry of Agriculture and farmer associations on diagnosing plant disease problems. Elite seed lines of tomato, cowpea and pumpkin were collected and preliminarily tested for disease resistance.